Temporal proteomic analysis of human cervicovaginal fluid with impending term labor

YJ Heng; MKW Di Quinzio; M Permezel; M Ayhan; GE Rice; HM Georgiou

Journal article

Temporal proteomic analysis of human cervicovaginal fluid with impending term labor

YJ Heng, MKW Di Quinzio, M Permezel, M Ayhan, GE Rice, HM Georgiou

Journal of Proteome Research | AMER CHEMICAL SOC | Published : 2010

DOI: 10.1021/pr900892f

Abstract

This study utilized 2D-PAGE and MALDI-ToF to investigate labor-associated temporal protein changes in the human cervicovaginal fluid (>25 kDa). Monocyte/neutrophil elastase inhibitor, squamous cell carcinoma antigen 1, annexin A3, collagen and albumin demonstrated differential expression prior to and/or during term labor. These findings provide further insight into the extracellular matrix remodeling of the cervix and overlying fetal membranes and may be useful to develop predictive tools for labor.

University of Melbourne Researchers

Megan Di Quinzio Author

Michael Permezel Author

Harry Georgiou Author

Related Projects (2)

IDENTIFICATION OF NOVEL BIOMARKERS PREDICTIVE OF IMPENDING PRETERM LABOUR

2007 DEVELOPMENT GRANT - (DEVELOPMENT OF A MULTIPLEX ASSAY FOR THE IDENTIFICATION OF WOMEN AT RISK OF PRETERM LABOUR)

Preterm birth (ie birth before the 37th week of gestation) is the most significant problem facing contemporary clinical obstetrics in the de..

Grants

Awarded by National Health and Medical Research Council of Australia (NHMRC) Development

Awarded by Postgraduate Research Scholarship

Funding Acknowledgements

The authors thank the Mercy Hospital for Women medical and midwifery staff for obtaining study samples, in particular, Valerie Bryant, Gabrielle Fleming, March Reeves and Anne Beeston. Karen Oliva provided technical assistance with the Ettan workstation. Microbiological assessments were performed by the Microbiology Department, Austin Patholop, Austin Health, Heidelberg, Victoria, Anstralia. This work was supported by a National Health and Medical Research Council of Australia (NHMRC) Development Grant (No. 454451) and the Medical Research Foundation for Women and Babies. Y.J.H. is a recipient of the National Health and Medical Research Council Biomedical (Dora Lush) Postgraduate Research Scholarship (No. 454880).